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Protein function

A practical approach

Szerkesztő
Oxford
Kiadó: IRL Press
Kiadás helye: Oxford
Kiadás éve:
Kötés típusa: Fűzött papírkötés
Oldalszám: 306 oldal
Sorozatcím: The Practical Approach Series
Kötetszám:
Nyelv: Angol  
Méret: 23 cm x 16 cm
ISBN: 0-19-963007-0
Megjegyzés: Fekete-fehér ábrákkal.
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Fülszöveg


Protein function: A Practical Approach concentrates on aspects of protein function that are common to the majority of proteins. It is a collection of methods that specifically demonstrate the detailed analyses of protein structure that can be carried out without highly specialized equipment.
Methods described here cover maintenance of protein function • measuring ligand—protein binding affinities • ligand blotting • affinity labelling • cross-linking to large ligands and between subunits • determination of subunit roles • analysis, identification and purification of sequence-specific DNA-binding proteins • chemical characterization of functional groups by competitive labelling • chemical modification • site-directed mutagenesis.
Using these protocols, researchers will be able to obtain a great deal of information about the functional properties of any protein. The book includes techniques which are somé of the most recent and efficient yet devised. A companion volume, Protein... Tovább

Fülszöveg


Protein function: A Practical Approach concentrates on aspects of protein function that are common to the majority of proteins. It is a collection of methods that specifically demonstrate the detailed analyses of protein structure that can be carried out without highly specialized equipment.
Methods described here cover maintenance of protein function • measuring ligand—protein binding affinities • ligand blotting • affinity labelling • cross-linking to large ligands and between subunits • determination of subunit roles • analysis, identification and purification of sequence-specific DNA-binding proteins • chemical characterization of functional groups by competitive labelling • chemical modification • site-directed mutagenesis.
Using these protocols, researchers will be able to obtain a great deal of information about the functional properties of any protein. The book includes techniques which are somé of the most recent and efficient yet devised. A companion volume, Protein Structure: A Practical Approach alsó edited by Thomas Creighton, provides the methods necessary for the study of protein structure. Vissza
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