Febs Letters Volume 59, Number 1-2./Volume 60, Number 1-2./Master Index Volumes 51-60.
1, 15 November, 1, 15 December 1975/Master Index Volumes 51-60 (March 1975-December 1975) - For the rapid publication of short reports in biochemistry, biophysics and molecular biology
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Volume 59, number 1
FEBS LETTERS
November 1975
Review Letter
ABSENCE OF A METABOLICALLY INDUCED ELECTRICAL POTENTIAL ACROSS THE MITOCHONDRIAL SEMIPERMEABLE MEMBRANE
Henry TEDESCHI...
Tovább
Előszó
Volume 59, number 1
FEBS LETTERS
November 1975
Review Letter
ABSENCE OF A METABOLICALLY INDUCED ELECTRICAL POTENTIAL ACROSS THE MITOCHONDRIAL SEMIPERMEABLE MEMBRANE
Henry TEDESCHI
Department of Biological Sciences, States University of New York, Albany, New York, U.S.A.
Received 10 August 1975 Revised version received 16 September 1975
It is not generally recognized that the results presented by Mitchell and Moyle [1] on the distribution of K'^ in isolated rat liver mitochondria in the presence of valinomycin constitute clear evidence for the absence of an electrogenic pump. Upon the initiation of metabolism, in the presence of valinomycin, the H'^ efflux corresponds to the uptake of K* (figs. 5-8, [1]). Under these conditions, the negative charge generated in the inner mitochondrial phase increases with time and corresponds quantitatively to the K'" taken up in the electroneutral exchange. At steady state, the internal negative charge, the corresponding high internal concentration of K'' and the relatively high permeability to K* are in harmony with a classical Gibbs-Donnan distribution (e.g., see [2] ) as it holds, for example, in muscle, (e.g., see [3] ). This situation quantitively explains the distribution of K* observed by Mitchell and Moyle [I ] and by others in subsequent studies (e.g. [4—6]). They are not consistent with the electrogenic pump model of the chemiosmotic hypothesis.
The principles involved can be most easily presented by an actual example. In the experiments of Mitchell and Moyle (figs.5 and 7 of [1 ]) the total efflux of H*^ corresponds to about 30 Meq./g mitochondrial protein and hence to the same amount of internal negative charge (X") balanced by a corresponding K* influx. Using the mitochondrial volume assumed by Mitchell and Moyle (0.4 X 10'^ 1/g mitochondrial protein), this amount corresponds to a concentration of 75 X 10"^ of X' equivalents/liter. The Donnan ratio if) or internal, over external K* concentration can be readily calculated using the X" concentration and the external concentration of K'^ (K'^o given by
Mitchell and Moyle in item 111 of their table ( (K^o = 3.4 X 10"" M), since.
(X-) 2(K^o
(Xf
(A)o (KOoJ
1/2
(1)
In Eqn [I ], (A)o is the external concentration of an anion which follows a Donnan distribution. Under the conditions of the experiments (A)o/(K'*)o is negligible and hence the Donnan ratio is expressed by Eqn 2.
/¦ = (X-)/(K\
(2)
The calculated value of r is approximately 220. The value used by Mitchell and Moyle to calculate a presumed potential of — 139 mV for these conditions is about 230. The calculation leaves no question that the experimentally obtained distribution is quantitatively explained in the absence of a membrane potential. Other results summarized in table 1 are consistent with this view. The observed distribution of Rb* and Ca^"^ in the presence of valinomycin should result in the same Donnan ratios for these ions as well, as in fad observed [8,9].
Paradoxically, in mitochondria in the presence of valinomycin, the higher internal K* concentration and the high K*^ permeability (in this case induced by valinomycin, see [5] ) should produce a K'^ diffusion potential, as it does in intact muscle (e.g., see [3] ). This potential should be approximated by the Nernst equation. A K* diffusion potential was suggested earlier [10] to explain, on the basis of the chemiosmotic hypothesis, the phosphorylation observed in
North-Holland Publishing Company ~ Amsterdam
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Vissza
Tartalom
Volume 59, number 1
1 November 1975
CONTENTS
Review Letter
Absence of a m'etabolically induced electrical potential across the mitochondrial semipermeable membrane,//. 7"edescft/
Research Letters
Mechanism of polyadenylate polymerase: formation of enzyme-substrate and
enzyme-primer complexes, V. S. Sethi Free and membrane-bound rabbit reticulocyte ribosomes. Proteins from the large
and the small subunits, M. Fehlmann, G. Bellemare, C. Godin The enzymatic reduction of K-vitamins incorporated in the membrane of liposomes, C. Martins, R. Ganser, A. Viviani The functional properties of sickle cell blood, L. Rossi-Bernardi, M. Luzzana, M.
M. Sama/a, F. Rossi, M. Perrella, R. L. Berger Light-activated rhodopsin phosphorylation may control light sensitivity in isolated rod outer segments, J. A. Miller, A. E. Brodle, M. Bownds The 5'-end groups of Alfalfa Mosaic Virus RNAs are m'G^'ppp^'Gp,/ Pinck Reversible uncoupling of the copper and cobalt spin systems in cobalt bovine superoxide dismutase at low pH, L. Calabrese, D. Cocco, L. Morpurgo,
B. Mondovi, G. Rotilio Preparation of active iodinated specific antibodies, J.-M. Pages, D. Louvard,
C. iMZdunski
Influence of the ionophore A 23 187 on myogenic cell fusion, Ch. Schudt,
D. Pette
Ca^^ transport across plasma and mitochondrial membranes of isolated
hepatocytes,,W. P. Dubinsky, R. S. Cockrell Computer analysis of conformational possibiUties of double-hehcal DNA,
K B. Zhurkin. Yu. P. Lysov, V. I. Ivanov Influence of electrostatic surface potential on mitochondrial ADP-phosphory-
lation, G. Schäfer, G. Rowohl-Quisthoudt Ascorbate as a natural constituent of chromaffin granules frorh the bovine
adrenal medulla, O. Terland, T. Flatmark A temary acetylchohne-manganese-ATP complex,/. Reuben Presence of a hypermodifled nucleotide in He La cell 18 S and
Saccharomyces carlsbergensis 17 S ribosomal RNAs, B. E. H. Maden, J. Forbes, P. deJonge.J. Klootwijk Identification of neighbouring proteins in E. coli 50S ribosomes, /I. Expert-
Bezanfon, D. Barritault, J. C. S. Clegg, M. Milet, Y. Khouvine, D. H. Hayes On the pH- and temperature dependence of the p-nitrophenylphosphatase
activity of intact Ehrlich ascites tumor cells, M. Loffler, F. Schneider Effects of cholinergic agents and sodium ions on the levels of guanosine and adenosine 3':5'-cyclic monophosphates in neuroblastoma and neuroblastoma X glioma hybrid cells, R. J. Gullis. J. Traber, K. Fischer, C. Buchen, B. Hamprecht Ornithine decarboxylase activity in germinating conidia oi Aspergillus
nidulans, L. Stevens Tissue specific differences in the 2'-0-methylation of eukaryotic 5.8S ribosomal RNA, R. N. Nazar, T. O. Sitz, H. Busch Thallium activation and inhibition of yeast aldehyde dehydrogenase, K. Bostian. G. F. Betts, W. K. Man, M. N. Hughes
(continued on page 3 of cover)
13
15
20 24
29
32
36
39
44
52 57
60
64
70
33[]]Sletters
Volume 59, number 1 1 November 1975
CONTENTS
(continued from page 4 of cover)
Enhancement by chelating agents of lead toxicity to mitochondria in the
presence of inorganic phosphate, D. R. Parr, E. J. Harris 92
The primary structure of protein L27 from the peptidyl-tRNA binding site
of Escherichia coii ribosomes, R. Chen. L. Mende, U. Arfsten 96
The isolation and characterization of glycopeptides and mucopolysaccharides from plasma membranes of normal and regenerating livers of rats, M. Akasaki, T. Kawasaki, I. Yamashina 100
The importance of the conformation of the tetrahedral intermediate for the
a-chymotrypsin-catalyzed hydrolysis of peptide substrates, S. A. Bizzozero, B.O.Zweifel 105
Tlie mechanism of K*-stimulated exogenous NADH oxidation in plant mitochondria, Miaz-nsftaw 109 Net synthesis of short RNA chains by E. eoli RNA polymerase at low pH,
U. Wienand, E. Fuchs 113
Isolation and identification of an actin from rat liver, V. M. Govindan,
Th. Wieland 117
p-Hydroxybenzoate synthase: a complex associated with mitochondrial membranes of roots of Cucumis sativus, P. Hagel, H. Kindl 120 Studies on a DNA polymerase 7-Iike enzyme from wheat embryos,
L. Tarrago-Litvak, M. Castroviejo, S. Liivak 125
The interaction of D and L-alanine with an optically active model meinbrane
system, A. S. Tracey, P. Diehl 131
Joint use of light, X-ray and neutron scattering for investigation of RNA and protein mutual distribution within the 50S subparticle of E. coii ribosomes,/. N. Serdyuk, A. K. Grenader 133
Volume 59, number 2 15 November 1975
CONTENTS
Discussion Letter
nie protonmotivc Q cycle: a general formulation, P. Mitchell 137
Review Letter
Albumin as tlie major metal transport agent in blood, F. Friedberg 140
Research Letters
Active and inactive forms of pyruvatedehydrogenase in skeletal muscle as related to tlie metabolic and functional state of tlie muscle cell,
G. Henning, G. Löffler, O. H. Wieland 142 A ferredoxin from Agrobacterium tubefaciens, J. van Beeumen, J. de Ley,
D. O. Hall, R. Cammack, K. K. Rao 146
The location of die major polypeptide of the ox heart mitochondrial inner
membrane,/).i?oxe/- 149
Proton-motive force in the obligately anaerobic bactcrium Qostridium
pasteurianum: a role in galactose and gluconate uptake,/. R. Booth, J.G.Morris " 153
The CIO, ion as a probe in n.m.r. studies of protein anion binding sites,
P. Reimarsson, T. Bull, B. Lindman 158
Cyanide generation from carbonylcyanide m-ciilorophenylliydrazone and illuminated grana or algae, E. K. Pistorius, H.-S. Gewitz, H. Voss, B. Vennesland 162
Purification, stability and inhibition of the collagenasc from Achromobacter iophagus, A. Lecroisey, V. Keil-Dlouha, D. R. Woods, D. Perrin, B.Keil 167
Tlie modification of tyrosyl residues in L-asparaginases and ribonuclease A with Fremy's salt. Implication of tyrosyl residues in the activity of L-asparaginases from E. coli B and Erwinia carotovora, R. B. Homer, S. R. Allsopp, J. E. Arrieta 173
Ribopolynucleotidcs modified at pyrimidine residues are cleaved selectively
by Tj ribonuclease at purine residucs,.4. M. Mazo, L. L. Kisselev 177
Effect of thenoyltrifluoroacetone on oxygen consumption and energy
conservation in isolated rat liver mitochondria, R. Ulvik, I Romslo. 180
Direct measurement of the protons pumped into tlie inner pliase of Üie functional membrane of photosynthesis per electron transfer, P. Gräber, H. T. Witt 184
Quantitative determination of neuraminic acid by gas chromatography - mass
spectrometry, L Mononen, J. Kärkkäinen 190
A high affinity receptor for indoleacetic acid in cultured tobacco pith explants,
H. Oostrom, M. A. van Loopik-Detmers, K. R. Libbenga 194 Subcellular localisation of iron during accelerated ferritin syntliesis,/. A. Millar 198 Cyanogen bromide fragments of the cardiac 1 light chain from bovine myosin:
evidence for sequence liomology with rabbit skeletal myosin alkali light chains, A. G. Weeds 203
Enhancement by immunoglobulin M of leukocytes during pliagocytosis,
T. Mizuochi, Y. Nishimura, A. Sakai, O. Takenaka, Y. Inada 209
(continued on page 3 of cover)
-SBiUSLETTERS
Volume 59, number 2
15 November 1975
CONTENTS
(continued from page 4 of cover)
Cerulcotoxin: an acidic neurotoxin from the venom o{ Bungarus caeruleus which blocks the response to a cholinergic agonist without binding to the cholinergic reccptor site, C. Bon, J.-P. Changeux 212
Primary structure of protein SI 8 from tlie small Escherichia coli ribosomal
subunit, M. Yaguchi 217
Fractionation of plant chromatin witli immobilized RNA-polymerase,
R. Jalouzot, M. Gontcharoff, D. Briane 221
Cytochrome P-450 associated with free polysome fractions,/. A. Craft,
M. B. Cooper, E. A. Shephard. B. R. Rabin 225
Acetylcarnitine metabolism in blowfiy flight muscle mitochondria, S. M. Danks,
J. B. Chappell 230
Benzoylglutamic acid, a metabolite of benzoic acid in Indian fruit bats, J. R. Idle,
P. Millburn, R. T. Williams 234
Inhibition of normal red cell glucose 6-phosphate dehydrogenase (G-6-PD) by a
variant enzyme, G-6-PD Hamburg, M. Gahr, W. Schröter 237
Tliermodynamic and binding properties of carboxymethylated cytochrome c,
S. C. Cooper, J. Lambek, M. Erecinska 241
Malignant transformation-linked imbalance: decreased xanthine oxidase activity
in hepatomas, N. Prajda, G. Weber 245
Oiromatin conformation as affected by shearing and by tlie removal of a portion
of non-histone proteins, T. Tashiro, M. Kurokawa 250
Kinetic mechanism of the (^^ P] ATP-PPj exchange reaction catalysed by yeast phenylalanyl-tRNA syntlietase, L L. Kisselev. F. Fasiolo, E. G. Malygin, V. V. Zinoviev 254
Magnetic enzyme membranes as active elements of electrochemical sensors. Specific amino acid enzyme electrodes, C. Calvot, A.-M. Berjonneau, G. Gellf, D. Thomas 258
Magnetic enzyme membranes as active elements of electrochemical sensors. Lactose, saccharose, maltose bienzyme electrodcs, M. Cordonnier, F. Lawny, D. Chapot, D. Thomas 263
Identification of the DCCD-reactive protein of the energy transducing
adenosinetriphosphatase complex from Escherichia coli, K. Altendorf, W. Zitzmann 268
Mucidin-resistant antimycin A-sensitive mitochondrial mutant oi Saccharomyces
cerevisiae, J. Subik 273
A function common to iron-enterochelin transport and action of colicins B, I,
V in Escherichia coli, K. Hantke, V. Braun 111
The binary code for protein-nucleic acid recognition with repulsive guanine:
application to tRNA case, V. /. Ivanov 282
Near neighbors of IF3 bound to 30S ribosomal subunits, J. van Duin,
C G. Kurland, J. Dondon, M. Grunberg-Manago 287
Purification of rat liver epoxide hydratase to apparent homogeneity,
P. Bentley, F. Oesch 291
Properties and amino acid composition of pure expoxide hydratase,
P. Bentley, F. Oesch, A. Tsugita 296
Biosynthesis of catalase T during oxygen adaptation Qi Saccharomyces
cerevisiae, P. Zimniak, E. Hartter, H. Ruis 300
Translation of tlie messenger RNA for rabbit uteroglobin in Xenopus oocytes,
M. Beato, D. Rungger 305
Neutral red, a rapid indicator for pH-changes in the inner phase of tliylakoids,
W. Ausländer, W. Junge 310
Differential radioactive labeling of an IIS globular form of acetylcholinesterase,
P. J. Morrod, D. G. Qark 316
Corrigendum and Erratum 321
Autiior index 323
I-
Volume 60, number 1 1 December 197s
CONTENTS
Hypothesis
The link between charge separation, proton movement and ATPase reactions,
R. N. Robertson, N. K. Boardman j
Research Letters
Acetoacetyl-CoA synthetase; a lipogenie enzyme in rat tissue, B. M. Buckley,
D. H. Williamson j
Respiratory chain linked ferricyanide reduction drives active transport in membrane vesicles from Bacillus subtilis, A. Bisschop, H. J. Sips, W. N. Konings 1 \
L-asparaginase entrapped in liposomes: preparation and properties, V. Fishman,
N. atri 17
Interaction of the basic copolymer - poly(ornithine, leucine) with normal and
transformed cells,/). Duksin, E. Katchalski-Katzir, L. Sachs 21
Characterization of the free form of murein-hpoprotein from the outer
membrane of Escherichia coli B/r, V. Braun, K. Hantke, U. Henning 26
The respiratory chain of Thiobaccillus ferro-oxidans: the reduction of
cytochromes by Fe^^and the prehminary characterization of rusticyanin, a novel 'blue' copper protein, J. G. Cobley, B. A. Haddock 29
A specific 1:1 G-Actin: DNAase I complex formed by the action of DNAase 1
on F-Actin, H. G. Mannherz, J. Barrington Leigh, R. Leberman, H. Pfrang 34 Preparation of gibberelUn affinity adsorbents, H. D. Knöfel, P. Müller, R. Kramell,
G. Sembdner 39
Respiration-linked proton translocation in Azotobacter vinelandii, A. J. Downs,
C. W. Jones 42 Isoelectric focusing of membrane proteins from spinach chloroplasts,
/. Novak-Hofer, P.-A. Siegenthaler 47
Inliibition of cytochrome-bg oxidation by KCN,//. Böhme 51
A transmembrane location for the proton-translocating reduced ubiquinone nitrate reductase segment of the respiratory chain.oi Escherichia coli,
D. H. Boxer, R. A Clegg 54 The binding of a spin-labelled dipeptide to papain, A. Kalk, A. S. H. de Jong,
M. D. Barratt 58
Cell surface effects of pokeweed observed by electrophoretic light scattering,
J. Josefowicz, F. R. Hallett 62
Yeast ribosomal sensitivity and resistance to the Amaryllidaceae alkaloids,
A. Jimenez, L. Sanchez. D. Vazquez 66
Lipid-protein interaction in porcine high-density (HDL,) lipoprotein, H. Hauser 71 The quantitation of glucosamine and galaetosamine in glycoproteins after
hydrolysis in p-toluenesulphonic acid, A. K. Allen, A. Neuberger 76
Myosin-linked calcium regulation in vascular smooth muscle, U. Mrwa, J. C. Ruegg 81 Repetitive primate DNA containing the recognition sequences for two
restriction endonucleases which generate cohesive ends, P. Gruss, G. Sauer 85 Nitrogenase oi Azotobacter chroococcum: inhibition by ADP of the reduction of oxidised Fe protein by sodium dithionite, M. G. Yates, R. N. F. Thorneley, D. J. Lowe 89
Hybridization of prokaryotic and eukaryotic 5S rRNA to Euglena gracilis
chloroplast DNA, D. O. Phillips, N. G. Carr 54
Deactivation of superoxide dismutase of EDTA-treated chloroplasts, R. Schmid 98 Antibody competition for plasma protein-bound estriol, J. D. Grant J. R. Soares,
S. J Gross 103
(continued on page 3 of cover)
Volume 60, number 1 1 December 1975
CONTENTS
(continued from page 4 of cover)
Ribosomal translocation promoted by guanylylimido diphosphate and guanylyl-
methylene diphosphonate, J. Modolell, T. Girbés, D. Vázquez 109
Rat liver soluble nuclear and cytoplasmic proteins with liigh affinity to polynucleotides,^. Schweiger, G. Mazur 114 Physical map of circular mitochondrial DNA from Neurospora crassa, V. Bernard,
A. Fühler, H. Küntzei 119 Functional and structural roles of sarcoplasmic reticulum protein components,
N. T. Malan, R. Sabbadini, D. Scales, G. Inesi 122
Binding of UDP-glucose to homogenates of normal and denervated skeletal muscle,
J. McLaughlin, M. N. Kucherov, H. Bruce Bosmann 126
Activation of glutamate dehydrogenase by 3', 5'-cyclic adenosine monopliosphate,
M. S. Setchenska, J. G. Vassilem-Popova, E. M. Russanov 129
Bovine pepsin: the sequence of the first 65 amino residues. (Completing the
sequence of the first 110 residues of bovine pepsinogen), M. K. Harboe,
B. Foltman 133 Prediction of the three-dimensional structure for ribosomal protein L25,
A. V. Finkelstein, S. A. Kozitsyn, O. B. Ptitsyn 137
The prosthetic group of myeloperoxidase, JV. C. Wu,J. Schultz 141
Origin of 5a-pregnane-3o;,20a,21-triol 3-sulphate in pregnant women,
T. A. Baillie, J. Sjovall, K. Sjovall 145
A lipoxygenase in rabbit reticulocytes which attacks phospholipids and intact
mitochondria, T. Schewe, W. Halangk, Ch. Hiebsch, S. M. Rapoport 149
The primary structure of protein L30 from Escherichia coli ribosomes,
L Ritter, B. Wittmann-Liebold 153
The subunits of yeast phenylalanyl-tRNA synthetase: a new fractionation
procedure based upon their cysteine contents, F. Fasiolo, Y. Boulanger 156
Persistence of thymine dimers in the replicated DNA oí Escherichia coli
B/r Sedliaková, J. Brozmanová, F. Masek 161
Nanosecond pulse fluorometry in polarized light of dansyl-L-cysteine linked to a unique SH group of F-actin; the influence of regulatory proteins and myosin moiety,P. Wahl, K. Mihashi, J.-C. Auchet 164
Involvement of the D-stem of tRNA*"''® (E. coli) in interaction with phenylalanyF tRNA synthetase as shown by chemical modification, V. N. Ankilova, V. V. Vlassov, D. G. Knorre, N. V. Melamed, N. A. Nuzdihna 168
Investigation of the phenylalanyl-tRNA synthetase modification with 7-(p-azido-anilide)-ATP, V. N. Ankilova, D. G. Knorre, V. V. Kravchento, O. /. Lavrik,
G. A. Nevinsky 172 K^ fluxes and the mitochondrial membrane potential, 7. J. Diwan, H. Tedeschi 176 The subunit composition of beef heart cytociuome c oxidase,
J. R. Bucher, R. Penniall 180
Spin labels for cells surfaces, J. R. Lepock, P. D. Morse II, R. J. Mehlhorn,
R. H. Hammerstedt, W. Snipes, A. D. Keith 185
Membrane-ribosome interactions: artefacts resulting from the temperature-
dependent formation of ribosomal aggregates, i". R. Mcintosh, R. P. Clark, B. R. Rabin 190
Binding and arrangement of non-histone proteins in chromatin-like structures
from mammalian cells, E. Jost, S. Qark 197
Comparison between the 3'OH end RNA sequence of two strains of tobacco mosaic virus (TMV) which may be aminoacylated, D. Lamy, G. Jonard,
H. Guillev, L Hirth 202 Low concentrations of C,-substituted purines retard the G» ^ G, transition of
bovine lens epithelium cells, D. Glässer, M. /wig 205
Salt response of ribosomes of a moderately haiophilic bacterium, R. Wydro,
M. Kogut, D. J. Kushner 210
Book Reviews and Book List No. 2 217
-aain^LETTERS
Volume 60, number 2
15 December 1975
CONTENTS
Review Letter
DNA polymerases of eukaryotes, A. M. Holmes. I. R. Johnston 233
Hypothesis
A hypothesis on membranous proteins specialized in lateral transport,
A. I. Archakov, A. V. Karyakin, V. P. Skulachev 244
Research Letters
The amino-terminal sequence of an invertebrate trypsin (crayfish A stacus leptodactylus): homology with other serine proteases, R. Zwilling. H. Neumth, L. H. Ericsson, D. L. Enfield 247
Masking of co-operativity of nucleotide sites in pig heart mitochondrial ATPase
(F, ) by heating, C. Godinot, A. Di Pietro. D. C. Gautheron 250
Stimulation of triacylglycerol synthesis by Z protein in rat liver and intestinal
mucosa,/'. J. A. O'Doherty, A. Kuksis 256
Studies on conformation and antigenicity of reduced 5-methylated
asparaginase in comparison with asparaginase, K. Todokoro. T. Salto, M. Obato, S. Yamazaki, Y. Tamaura, Y. Inada 259
Differences in the carbohydrate portion of the a subunit of porcine luttopin (LH), foUitropin (FSH) and thyrotropin (TSH), G. Maghuin-Rogister, J. Closset, G. Hennen 263
The oxygen dissociation curve of viper (Viper áspis) hemoglobin: functional
similarity with human hemoglobin Portland, M. Duguet, R. Acher 267
Control of pyruvate dehydrogenase interconversion by palmitoyl-coenzyme A - as related to adenine nucleotide translocation in isolated fat cell mitochondria, G. Löffler, S. Bard, O. H. Wieland 269
Picosecond kinetics of the 1250 nm band of the Rps. sphaeroides reaction center: the nature of the primary photochemical intermediary state, P. L. Dutton. K. J. Kaufmann, B. Chance, P. M. Rentzepis 275
Linkage-specific a-D-galactosidases from Trichomonas foetus: characterisation of the blood-group B-destroying enzyme as a 1,3-a-galactosidase and the blood-group P,-destroying enzyme as a 1,4-a-galactosidase, A. D. Yates, W. T. J Morgan, W. M. Watkins 281
The primary structure of tRNA'^^e fj-om Bacillus stearothermophilus,
C. Guerrier-Takada. G. Dirheimer, H. Grosjean, G. Keith 286
Intercellular adhesion of neuroretina chick embryo cells: enhancement by
bovine serum albumin and derivatives, M. Cornet, F. Alliot, B. Pessac. M. Monsigny 290
Heterogeneity of cytochrome/'-450 in rat liver microsomes: selective interaction of metyrapone and SKF 525-A with different fractions of microsomal cytochrome/'¦450,//. Grasdalen, D. Bäckström, L. E. G. Eriksson, A. Ehrenberg, P. Moldáis, C. von Bahr, S. Orrenius 294
The amino acid sequence of gnu pancreatic ribonuclease, G. Groen, G. W. Welling,
J. J. Beintema 300
On the presence of pantothenic acid in the three complementary enzymes of
bacitracin synthetase,/. Roland. 0. Frifyshov. S. G. Laland 305
Tlie use of a homologous series of affinity labeling reagents in tiie study of the
biotin transport system in yeast, E. A. Bayer, T. Viswanatha, M. Wilchek 309 Solid phase Edman degradation. High yield attachment of tryptic protein fragments to aminated supports, P. Herbrink, G. I. Tesser,
J. J. M. Lamberts 313
Biosynthese de cellulose bacterienne a partir de glucose sélectivement
deuterié en position 6: étude par RMN, D. Gagnaire, F. R. Taravel 317
(continued on page 3 of cover)
BBIUSletters
Volume 60, number 2 15 December 1975
CONTENTS
ondnued from page 4 of cover)
Structure of the vasoactive intestinal octacosapeptide from chicken intestine.
The amino acid sequence, A. Nilsson 322
The anomeric configuration of iV-acetylneuraminic acid released by the action of
Vibrio cholerae neuraminidase, L. Holmquist, B. Ost man 327
The effect of pH of the midpoint oxidation-reduction potentials of components
associated with plant photosystem II, D. B. Knaff 331
Erythrocyte glyoxalase 1 polymorphism in an African and English population,
I. A. Bagster, C. W. Parr, S. G. Welch 336
A test for the active intermediate in the aminoacylation of tRNA^^® from yeast,
R. Hirsch, R. Thiebe 338
A new active intermediate in the aminoacylation of tRNA, R. Thiebe 342
Rapid release of free fatty acids during ceU breakage and their effects on a sugar-
proton cotransport system in Chlorella vulgaris, M. Decker, W. Tanner 346
The degradation of rRNA in ribosomes by ribonucleases present in rat liver
sub-cellular fractions, P. R. Mcintosh, B. R. Rabin 349
E.s.r. determination of Mn"^ uptake and binding in mitochondria,
M. Bragadin, P. DelVAntone, T. Pozzan, O. Vol pato, G. F. A z zone 354
The constituent polypeptide chain of porcine thyroglobulin, S. Lissitzky,
J. Mauchamp, J. Reynaud, M. Rolland 359
Interaction of purified human transcortin with spin labelled steroids, M. Basset,
G. Defaye, E. M. Chambaz 364 SV 40 nucleoprotein complexes: structural modifications after isopycnic
centrifugation in metrizamide gradients, P. F. Pignatti, C. Cremisi, O. Croissant, M. Yaniv 369
Isolation and chemical composition of the NADH: semidehydroascorbate oxidoreductase (EC 1.6.5.4)-rich membranes from rat liver, i). Geiss,
H.-U. Schulze 374 Glutamine cognate codons in rabbit haemoglobin mRNAs, K. Hilse, E. Rudloff 380 Purine salvage in mammary glands of mice, J. Barankiewicz, M. M. Jezewska,
P. Chomczynski 384
Konformationsänderungen vor und nach DNS-Synthese verstärken zellgenetisch
letale Strahlen schaden, G. Hagemann, J. Meilmann 388
Conjugation of foreign compounds in the elephant and hyaena, J. Caldwell,
M. R. French, J. R. Idle. A. G. Renwick, O. Bassir, R. T Williams 391
Early glucocorticoid-dependent stimulation of RNA polymerase B in rat
thymus cells, N. M. Borthwick, P. A. Bell 396
The formation oi Helix pomatia methaemocyanin accelerated by azide and
fiuoride, R. Witters, R. Lontie 400
Evidence that the temperature-dependent association of ribosomes with smooth microsomes determined by centrifugal separation may be an artefact, P. R. Mcintosh, R. P. Clark, B. R. Rabin 404
Analysis of the crystallinity of calcium phosphate deposits in rat liver
mitochondria by electron spin resonance spectroscopy,/T. Ostrowski, A. Dziedzic-Goclawska, A. Sliwowski, L. Wojtczak, J. Michalik, W. Stachowicz 410 Modification of the essential carboxyl group in octopine dehydrogenase,
C Hue, A. Olomucki, F. Thome-Beau 414
Perturbation of lipid structures by fluorescent probes, K. S. Krishnan, P. Balaram 419 Formation and enzymic properties of dimeric RNase P, K. R. Bärwald,
R. E. Reid, B. Gutte 423
The stoichiometry of polypeptide chains in the pyruvate dehydrogenase
multienzyme complex of E. coli determined by a simple novel method, D. L. Bates. R. A. Harrrison, R. N. Perham (i^jJ
Effect of fasting on the rates of lactate turnover and oxidation in rats,
A. Freminet, L. Leclerc, M. Gentil, C Poyart 431
Hb Newcastle: i392(F8)His ^ Pro, R. Finney, R. Casey, H. Lehmann, W. Walker 435 Autiior Index 439
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